Different methods and culture media were used on the same semen samples. Ejaculated and treated samples were evaluated by means of multiple exposure photography (MEP) in order to study changes in sperm motility. A statistical analysis of the normal sperm population distribution curve was done with two objectives: (1) evaluating semen improvement after treatment; and (2) finding out whether the post-treatment sample differed from the original one. When variables were analysed in the individual subject, a significant percentage improvement of mean velocity was observed. Only two methods improved the percentage of motile sperm. The influence of treatment and of culture media on sperm responses was also tested and a significant influence of the media was found (Ham F10 showed the best responses). No significant differences between the ejaculated and treated samples were found when mean velocity, percentage of motile sperm, variance population, and kurtosis were analysed, as grouped treatment variables.