Presence of corazonin in three insect species, and isolation and identification of [His7]corazonin from Schistocerca americana

Peptides. Nov-Dec 1991;12(6):1285-9. doi: 10.1016/0196-9781(91)90208-7.


An ELISA for corazonin, a cardioactive neuropeptide from the American cockroach, Periplaneta americana, was developed. It was used to isolate corazonin from the cockroach Nauphoeta cinerea, the locust Schistocerca americana, and the hawkmoth Manduca sexta. The peptides from Nauphoeta and Manduca had the same retention times as Periplaneta corazonin, and their amino acid compositions also suggested that these peptides are identical with corazonin. The corazonin-immunoreactive peptide from Schistocerca eluted slightly earlier on HPLC than corazonin, and its structure was determined to be [His7]corazonin, or pGlu-Thr-Phe-Gln-Tyr-Ser-His-Gly-Trp-Thr-Asn-amide. These results indicate that corazonin is generally present in insects and that its structure has been well conserved.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cockroaches / chemistry
  • Enzyme-Linked Immunosorbent Assay
  • Grasshoppers / chemistry
  • Insect Proteins*
  • Insecta / chemistry*
  • Molecular Sequence Data
  • Moths / chemistry
  • Neuropeptides / chemistry
  • Neuropeptides / isolation & purification*
  • Species Specificity


  • Insect Proteins
  • Neuropeptides
  • corazonin protein, insect