PON1 activity and total oxidant status in patients with active pulmonary tuberculosis

Clin Biochem. 2008 Feb;41(3):140-4. doi: 10.1016/j.clinbiochem.2007.11.018. Epub 2007 Dec 7.

Abstract

Objectives: The aim of this study was to the investigate effect of tuberculosis infection on paraoxonase-1 (PON1) activity and oxidative status in patients with pulmonary tuberculosis (PTB).

Design and methods: Twenty-five active PTB subjects and 33 healthy controls were included in the study. Serum PON1 activity, total oxidant status (TOS), lipid hydroperoxide (LOOH) and total free sulfydryl (-SH) groups were determined.

Results: Serum basal/salt-stimulated paraoxonase activities, arylesterase activity and total -SH group levels were significantly lower in patients with PTB than controls (p<0.05, p<0.05, p<0.001 and p<0.01 respectively), while TOS and LOOH levels were significantly higher (p<0.01 and p<0.05, respectively). In PTB patients, TOS, LOOH and total -SH group levels were significantly correlated with paraoxonase (r=-0.371, p<0.05; r=-0.286, p<0.05; r=0.625 p<0.01; respectively) and arylesterase (r=-0.437, p<0.01; r=-0.352, p<0.05; r=0.653, p<0.01; respectively).

Conclusions: Patients with active PTB are exposed to potent oxidative stress and they have decreased PON1 activity. These predisposal factors may, in part, play a role in the pathogenesis of atherosclerosis in PTB.

MeSH terms

  • Adult
  • Aryldialkylphosphatase / blood*
  • Atherosclerosis / blood
  • Atherosclerosis / etiology
  • Female
  • Humans
  • Lipid Peroxides / blood
  • Male
  • Middle Aged
  • Oxidants / blood*
  • Oxidation-Reduction*
  • Oxidative Stress*
  • Reference Values
  • Sulfhydryl Compounds / blood
  • Tuberculosis, Pulmonary / blood*
  • Tuberculosis, Pulmonary / complications*

Substances

  • Lipid Peroxides
  • Oxidants
  • Sulfhydryl Compounds
  • Aryldialkylphosphatase