[Immunorestitutive action of hydrolysates and ultrafiltrates of bovine spleen]

Arzneimittelforschung. 1991 Dec;41(12):1281-5.
[Article in German]

Abstract

Investigations with different spleen extracts of varied composition and purity have shown immunomodulatory effects. In the present study the in vivo effect of a bovine spleen hydrolysate (Prosplen) on a radiation-induced immunodeficiency model is demonstrated. Intraperitoneal application of 1 mg/g bw hydrolysate 3/times a week caused a highly significant acceleration of the restitution of the humoral and cell-mediated immunoreactivity towards sheep erythrocytes in sublethally (600 cGy) radiated mice: plaque-forming cells in the spleen, hemagglutination, hemolysistiter of the serum, delayed type of hypersensitivity = DTH reaction. The immunoreactivity of the treated groups reached normal levels within 6-8 weeks, whereas in the groups receiving placebo this was observed after 10 weeks at the earliest. Cytofluorometric investigations revealed an accelerated restitution of the B-lymphocyte fraction in the spleen. Mice that was not irradiated showed no effect after treatment with the hydrolysate. Similar results were obtained using an ultrafiltrate of the hydrolysate.

Publication types

  • English Abstract

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Antibody Formation / drug effects
  • Cattle
  • Cell Division / drug effects
  • Filtration
  • Flow Cytometry
  • Hemagglutination Tests
  • Hemolysis / drug effects
  • Hemolytic Plaque Technique
  • Hydrolysis
  • Hypersensitivity, Delayed / immunology
  • Immunity, Cellular / drug effects
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Radiation Injuries, Experimental / immunology
  • Sheep / immunology
  • Spleen / physiology*
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology
  • Tissue Extracts / pharmacology*

Substances

  • Adjuvants, Immunologic
  • Tissue Extracts