Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach

Gordon Stevenson; Manuela Dieckelmann; Peter R Reeves

doi:10.1128/AEM.02660-07

Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach

Appl Environ Microbiol. 2008 Feb;74(4):1294-8. doi: 10.1128/AEM.02660-07. Epub 2007 Dec 21.

Authors

Gordon Stevenson¹, Manuela Dieckelmann, Peter R Reeves

Affiliation

¹ School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia.

Abstract

We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-antigen GT genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Escherichia coli / enzymology*
Escherichia coli / genetics*
Glucosyltransferases / genetics
Glucosyltransferases / metabolism*
Models, Biological
Multigene Family
O Antigens / genetics
O Antigens / metabolism*
Oligonucleotides / genetics
Species Specificity
Substrate Specificity

Substances

O Antigens
Oligonucleotides
Glucosyltransferases