A positive association of lumbar spine bone mineral density with dietary protein is suppressed by a negative association with protein sulfur

J Nutr. 2008 Jan;138(1):80-5. doi: 10.1093/jn/138.1.80.


Dietary protein is theorized to hold both anabolic effects on bone and demineralizing effects mediated by the diet acid load of sulfate derived from methionine and cysteine. The relative importance of these effects is unknown but relevant to osteoporosis prevention. Postmenopausal women (n = 161, 67.9 +/- 6.0 y) were assessed for areal bone mineral density (aBMD) of lumbar spine (LS) and total hip (TH) using dual X-ray absorptiometry, and dietary intakes of protein, sulfur-containing amino acids, and minerals using a USDA multiple-pass 24-h recall. The acidifying influence of the diet was estimated using the ratio of protein:potassium intake, the potential renal acid load (PRAL), and intake of sulfate equivalents from protein. aBMD was regressed onto protein intake then protein was controlled for estimated dietary acid load. A step-down procedure assessed potential confounding influences (weight, age, physical activity, and calcium and vitamin D intakes). Protein alone did not predict LS aBMD (P = 0.81); however, after accounting for a negative effect of sulfate (beta = -0.28; P < 0.01), the direct effect of protein intake was positive (beta = 0.22; P = 0.04). At the TH, protein intake predicted aBMD (beta = 0.18; P = 0.03), but R2 did not improve with adjustment for sulfate (P = 0.83). PRAL and the protein:potassium ratio were not significant predictors of aBMD. Results suggest that protein intake is positively associated with aBMD, but benefit at the LS is offset by a negative impact of the protein sulfur acid load. If validated experimentally, these findings harmonize conflicting theories on the role of dietary protein in bone health.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aged
  • Bone Density / drug effects*
  • Bone Density / physiology
  • Bone and Bones / physiology
  • Dietary Proteins / administration & dosage
  • Dietary Proteins / pharmacology*
  • Female
  • Humans
  • Lumbar Vertebrae / drug effects*
  • Lumbar Vertebrae / physiology*
  • Middle Aged
  • Osteoporosis / diagnosis
  • Postmenopause / physiology
  • Sulfur / analysis*


  • Dietary Proteins
  • Sulfur