Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains

AIDS Res Hum Retroviruses. 2007 Dec;23(12):1593-8. doi: 10.1089/aid.2007.0130.


The HIV fusion inhibitor enfuvirtide (ENF/Fuzeon) targets the env gp41 transmembrane domain. Mutations in gp41 are associated with ENF resistance. We developed a prototype assay to genotype a 676-bp region spanning the heptad repeat domains (HR1 and HR2) of HIV-1 gp41. Plasma samples were collected from 126 HIV-1-infected blood donors in Cameroon, Brazil, Uganda, South Africa, Thailand, and Argentina. Based on analysis of gag p24, pol integrase, and env gp41 genes, the panel was composed of subtypes A/A2 (18), B (11), C (14), D (10), F/F2 (9), G (7), CRF01_AE (9), CRF02_AG (33), and recombinant strains (15). Genotyping was successful for 119 of the 126 samples (94.4%). Although numerous amino acid polymorphisms were detected in some samples, none had primary mutations associated with ENF resistance. The gp41 HIV-1 research reagents developed by Celera are useful tools for genotyping analysis of the gp41 region in diverse HIV-1 strains.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Base Sequence
  • Drug Resistance, Viral
  • Enfuvirtide
  • Genotype
  • HIV Envelope Protein gp41 / chemistry
  • HIV Envelope Protein gp41 / genetics*
  • HIV Envelope Protein gp41 / pharmacology
  • HIV Fusion Inhibitors / pharmacology
  • HIV Infections / virology*
  • HIV-1 / drug effects
  • HIV-1 / genetics*
  • Humans
  • Molecular Sequence Data
  • Peptide Fragments / pharmacology
  • Polymorphism, Genetic*
  • Protein Structure, Tertiary
  • Sequence Alignment


  • HIV Envelope Protein gp41
  • HIV Fusion Inhibitors
  • Peptide Fragments
  • Enfuvirtide