Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp

J Am Chem Soc. 2008 Jan 23;130(3):784-5. doi: 10.1021/ja0759139. Epub 2008 Jan 1.


We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / metabolism*
  • Fluorescence
  • Fluorescent Dyes / chemistry*
  • Guanosine Pentaphosphate / analysis*
  • Pyrenes / chemistry*
  • Pyridines / chemistry*
  • Water / chemistry


  • (1-((pyren-1-yl)propoxy)-3,5-bis(bis(2-pyridylmethyl)aminomethyl)benzene)zinc(II)
  • Fluorescent Dyes
  • Pyrenes
  • Pyridines
  • Water
  • Guanosine Pentaphosphate