Molecular mechanisms of oxidative stress resistance induced by resveratrol: Specific and progressive induction of MnSOD

Biochem Biophys Res Commun. 2008 Mar 7;367(2):406-12. doi: 10.1016/j.bbrc.2007.12.138. Epub 2007 Dec 31.


trans-Resveratrol (3,4',5-trihydroxystilbene; RES), a polyphenol found in particularly high concentrations in red wine, has recently attracted intense interest for its potentially beneficial effects on human health. Here, we report the effects of long-term exposure to micromolar concentrations of RES on antioxidant and DNA repair enzyme activities in a human cell line (MRC-5). RES had either no effect on, or reduced the activities of glutathione peroxidase, catalase and CuZn superoxide dismutase (SOD), in treatments lasting up to 2 weeks. RES failed to induce activities of the DNA base excision repair enzymes apurinic/apyrimidinic endonuclease and DNA polymerase beta. However, it dramatically and progressively induced mitochondrial MnSOD expression and activity. Two weeks exposure to RES increased MnSOD protein level 6-fold and activity 14-fold. Thus, long-term exposure of human cells to RES results in a highly specific upregulation of MnSOD, and this may be an important mechanism by which it elicits its effects in human cells.

MeSH terms

  • Antioxidants / administration & dosage
  • Cell Line
  • Deoxyribonuclease (Pyrimidine Dimer) / metabolism*
  • Dose-Response Relationship, Drug
  • Escherichia coli Proteins / metabolism*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism*
  • Humans
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology*
  • Resveratrol
  • Stilbenes / administration & dosage*
  • Superoxide Dismutase / metabolism*
  • Up-Regulation / drug effects


  • Antioxidants
  • Escherichia coli Proteins
  • Stilbenes
  • Superoxide Dismutase
  • Deoxyribonuclease (Pyrimidine Dimer)
  • NTH protein, E coli
  • Resveratrol