Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies

Nat Methods. 2008 Feb;5(2):163-5. doi: 10.1038/nmeth1170. Epub 2008 Jan 6.


We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatin Immunoprecipitation / methods*
  • Chromosome Mapping / methods*
  • Epitope Mapping / methods*
  • Epitopes / genetics
  • Epitopes / immunology
  • Epitopes / metabolism
  • Genetic Vectors / genetics*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Proteins / genetics*
  • Proteins / immunology
  • Proteins / metabolism*
  • Staining and Labeling


  • Epitopes
  • Proteins