Trimethylsilyl-O-methyloxime derivatives for the measurement of [6,6-2H2]-D-glucose-enriched plasma samples by gas chromatography-mass spectrometry

J Chromatogr. 1991 Dec 6;572(1-2):302-6. doi: 10.1016/0378-4347(91)80494-w.


A new method for the determination of the enrichment of [6,6-2H2]-D-glucose in human plasma by gas chromatography-mass spectrometry (GC-MS) is described. (2,3,4,5,6)-Pentakis-O-trimethylsilyl-O-methyloxime-D-glucose is used as a derivative for the GC measurement. Using GC-MS with electron-impact ionization, the enrichment is measured in the single-ion monitoring mode observing the masses m/z 319 and 321. In contrast to other methods the use of this glucose derivative reduced the amount of plasma needed from 200 to 10 microliters and no chemical ionization equipment is needed for the mass spectrometer.

MeSH terms

  • Blood Glucose / analysis*
  • Deuterium / chemistry
  • Gas Chromatography-Mass Spectrometry
  • Glucose / analogs & derivatives*
  • Glucose / chemistry*
  • Humans
  • Oximes / chemistry*
  • Trimethylsilyl Compounds / chemistry*


  • Blood Glucose
  • Oximes
  • Trimethylsilyl Compounds
  • Deuterium
  • Glucose