Two-dimensional Polyacrylamide Gel Electrophoresis of Proteins Synthesized During Early Germination of Bacillus Subtilis 168 in the Presence of Actinomycin D

J Basic Microbiol. 1991;31(6):429-36. doi: 10.1002/jobm.3620310607.

Abstract

Two-dimensional gel electrophoresis was used to demonstrate the synthesis of approximately 65 [35S]-methionine-labelled soluble proteins between 0 and 10 min after the start of germination, of approximately 210 proteins at 10-20 min, and of approximately 260 proteins during vegetative growth of Bacillus subtilis. When actinomycin D and [35S]-methionine were administered at the onset of germination and the proteins synthesized during the subsequent 15 min were analyzed, two proteins were detected, and were designated protein I and protein II. Immunoblot analysis with an antiserum raised against RNA polymerase from Escherichia coli demonstrated that protein II corresponded to the sigma A factor of Bacillus subtilis. Thus, the sigma A factor is synthesized during early germination of Bacillus subtilis in the presence of actinomycin D.

Publication types

  • Comparative Study

MeSH terms

  • Bacillus subtilis / drug effects
  • Bacillus subtilis / metabolism*
  • Bacterial Proteins / biosynthesis*
  • Cell Division / physiology
  • DNA-Directed RNA Polymerases / immunology
  • Dactinomycin / pharmacology*
  • Electrophoresis, Gel, Two-Dimensional
  • Escherichia coli / metabolism
  • Immunodiffusion
  • Sigma Factor / biosynthesis
  • Spores, Bacterial / drug effects
  • Spores, Bacterial / metabolism*

Substances

  • Bacterial Proteins
  • Sigma Factor
  • Dactinomycin
  • DNA-Directed RNA Polymerases