CARM1 promotes adipocyte differentiation by coactivating PPARgamma

EMBO Rep. 2008 Feb;9(2):193-8. doi: 10.1038/sj.embor.7401151. Epub 2008 Jan 11.


The coactivator-associated arginine methyltransferase 1 (CARM1) is recruited to gene promoters by many transcription factors. To identify new pathways that use CARM1, we carried out a comprehensive transcriptome analysis of CARM1-knockout embryos. By using complementary DNA microarrays and serial analysis of gene expression, we identified various genes involved in lipid metabolism that were underrepresented in CARM1-knockout embryos, indicating an important role for this coactivator in adipose tissue biology. We also observed that the amount of brown fat in CARM1-knockout embryos is reduced. Furthermore, cells lacking CARM1 have a severely curtailed potential to differentiate into mature adipocytes. Reporter experiments and chromatin immunoprecipitation analysis show that CARM1 regulates these processes by acting as a coactivator for peroxisome proliferator-activated receptor gamma (PPARgamma). Together, these results show that CARM1 promotes adipocyte differentiation by coactivating PPARgamma-mediated transcription and thus might be important in energy balance.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology*
  • Adipocytes / drug effects
  • Adipocytes / enzymology*
  • Adipose Tissue, Brown / cytology
  • Adipose Tissue, Brown / drug effects
  • Adipose Tissue, Brown / embryology
  • Adipose Tissue, Brown / enzymology
  • Animals
  • Cell Differentiation* / drug effects
  • Embryo, Mammalian / drug effects
  • Embryo, Mammalian / enzymology
  • Estrogens / pharmacology
  • Gene Expression Profiling
  • Mice
  • PPAR gamma / metabolism*
  • Protein-Arginine N-Methyltransferases / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Transcription, Genetic / drug effects


  • Estrogens
  • PPAR gamma
  • RNA, Messenger
  • Protein-Arginine N-Methyltransferases
  • coactivator-associated arginine methyltransferase 1