Activity of adenosine deaminase and adenylate deaminase on adenosine and 2', 3(')-isopropylidene adenosine: role of the protecting group at different pH values

Nucleosides Nucleotides Nucleic Acids. 2008 Jan;27(1):31-6. doi: 10.1080/15257770701571776.

Abstract

The deamination rate of 2',3'-isopropylidene adenosine catalyzed by adenosine deaminase (ADA) from calf intestine and adenylate deaminase (AMPDA) from Aspergillus species has been evaluated and compared with that of the enzymatic reactions of adenosine, to elucidate the influence of the protecting group on enzyme activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AMP Deaminase / chemistry
  • AMP Deaminase / metabolism*
  • Adenosine / chemistry*
  • Adenosine Deaminase / chemistry
  • Adenosine Deaminase / metabolism*
  • Aspergillus / enzymology
  • Catalysis
  • Deamination
  • Hydrogen-Ion Concentration

Substances

  • Adenosine Deaminase
  • AMP Deaminase
  • Adenosine