Expression of coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy

J Pathol. 2008 Mar;214(4):415-24. doi: 10.1002/path.2308.


Coronin-3 (coronin-1C), a homotrimeric F-actin binding protein, has been shown to be important for cell migration and brain morphogenesis. Here, we present for the first time a detailed analysis of the expression pattern of coronin-3 in human brain tumours and demonstrate that coronin-3 expression correlates with malignant phenotype in diffuse gliomas. In general, the expression of coronin-3 varies in different brain tumour entities. However, in diffuse gliomas, the number of coronin-3 expressing tumour cells correlates with the degree of malignancy. High-grade gliomas, such as anaplastic astrocytomas, anaplastic oligodendrogliomas, anaplastic oligoastrocytomas and glioblastomas, show high numbers of tumour cells positive for coronin-3, while diffuse low-grade gliomas, such as diffuse astrocytomas, oligodendrogliomas and oligoastrocytomas, exhibit low numbers of coronin-3-positive tumour cells. In order to explore and verify a contribution of coronin-3 to the malignant phenotype of diffuse gliomas, we employed an efficient shRNA-mediated coronin-3 knockdown in U373 and A172 human glioblastoma cells. Coronin-3 knockdown glioblastoma cells exhibited reduced levels of cell proliferation, cell motility and invasion into extracellular matrix compared to control cells. Together, our findings demonstrate evidence for a contribution of coronin-3 expression in the malignant progression of diffuse gliomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Astrocytes / metabolism
  • Biomarkers, Tumor / deficiency
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism*
  • Biomarkers, Tumor / physiology
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / pathology
  • Cell Movement
  • Cell Proliferation
  • Extracellular Matrix / pathology
  • Glioma / metabolism*
  • Glioma / pathology
  • Humans
  • Matrix Metalloproteinases / metabolism
  • Microfilament Proteins / deficiency
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Microfilament Proteins / physiology
  • Neoplasm Invasiveness
  • Neoplasm Proteins / deficiency
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism
  • Neoplasm Proteins / physiology
  • Tumor Cells, Cultured


  • Biomarkers, Tumor
  • Microfilament Proteins
  • Neoplasm Proteins
  • coronin proteins
  • Matrix Metalloproteinases