Vocal quality was analyzed in 20 subjects who underwent total laryngectomy with insertion of voice prosthesis (TL with VP) and in 10 subjects who underwent supracricoid laryngectomy (SCL). Acoustic analysis was carried out using Lingwaves and Praat, for sustained vowels and conversation to obtain the fundamental frequency (F(0)) and jitter parameters. Perceptual evaluations and self-assessment of voice impact on the quality of life were also performed. Results showed statistically significant differences between TL+VP and SCL individuals for the mean F(0) during conversation, for Roughness, Grade of voice quality, and self-assessment of speech, with worst results in the SCL group.