Interleukin-27 upregulates major histocompatibility complex class II expression in primary human endothelial cells through induction of major histocompatibility complex class II transactivator

Hum Immunol. 2007 Dec;68(12):965-72. doi: 10.1016/j.humimm.2007.10.004. Epub 2007 Nov 20.


Interleukin-27 (IL-27) is a novel IL-12 family member that plays a critical role in the regulation of T-cell responses. Its immunoregulatory effects on endothelial cells (EC) remain unexplored. Here we show a role for IL-27 in the induction of major histocompatibility complex (MHC) expression in primary human EC. Stimulation of human umbilical vein ECs by IL-27 rapidly induces IFN regulatory factor-1 and dramatically increases the expression of major histocompatibility class II transactivator (CIITA) isoform IV. Expression of this transactivator correlates with increased MHC class II gene expression. IL-27 also enhances expression of MHC class I molecules. Furthermore expression of beta2-microglobulin and transporter associated with antigen processing-1 transcripts increases in response to IL-27. Additional microarray analysis demonstrates that IL-27 significantly upregulates a panel of genes that correlates with immune regulation, including the chemokines CXCL9, CXCL10, and CX3CL1 in human umbilical vein ECs. This first demonstration that both MHC II and I expression are increased in EC after IL-27 stimulation suggests that IL-27 may be important in conferring immune function on vascular endothelium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 2
  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / metabolism
  • Cells, Cultured
  • Chemokines / genetics
  • Chemokines / immunology
  • Chemokines / metabolism
  • Endothelial Cells / immunology*
  • Endothelial Cells / metabolism
  • Gene Expression Regulation*
  • Genes, MHC Class I
  • Genes, MHC Class II
  • Histocompatibility Antigens Class II / immunology
  • Histocompatibility Antigens Class II / metabolism*
  • Humans
  • Interferon Regulatory Factor-1 / immunology
  • Interferon Regulatory Factor-1 / metabolism*
  • Interleukin-17 / immunology
  • Interleukin-17 / metabolism*
  • Nuclear Proteins / immunology
  • Nuclear Proteins / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Trans-Activators / immunology
  • Trans-Activators / metabolism*
  • Umbilical Veins / cytology
  • Up-Regulation
  • beta 2-Microglobulin / genetics
  • beta 2-Microglobulin / immunology
  • beta 2-Microglobulin / metabolism


  • ATP Binding Cassette Transporter, Subfamily B, Member 2
  • ATP-Binding Cassette Transporters
  • Chemokines
  • Histocompatibility Antigens Class II
  • IRF1 protein, human
  • Interferon Regulatory Factor-1
  • Interleukin-17
  • MHC class II transactivator protein
  • Nuclear Proteins
  • RNA, Messenger
  • TAP1 protein, human
  • Trans-Activators
  • beta 2-Microglobulin