Abstract
An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential beta-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Archaeal Proteins / genetics
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Archaeal Proteins / metabolism
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Base Sequence
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Gene Expression Regulation, Archaeal
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Genes, Archaeal / genetics*
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Glycoside Hydrolases / genetics
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Glycoside Hydrolases / metabolism
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Molecular Sequence Data
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Oligonucleotide Array Sequence Analysis / methods
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Operon / genetics*
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Thermococcus / genetics*
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Transcription, Genetic*
Substances
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Archaeal Proteins
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Glycoside Hydrolases