Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey

Carbohydr Res. 2008 Mar 17;343(4):651-9. doi: 10.1016/j.carres.2007.12.011. Epub 2008 Jan 14.


Using HPLC a fraction of New Zealand manuka honey has been isolated, which gives rise to the non-peroxide antibacterial activity. This fraction proved to be methylglyoxal, a highly reactive precursor in the formation of advanced glycation endproducts (AGEs). Methylglyoxal concentrations in 49 manuka and 34 non-manuka honey samples were determined using a direct detection method and compared with values obtained using standard o-phenylenediamine derivatisation. Concentrations obtained using both the methods were similar and varied from 38 to 828 mg/kg.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / isolation & purification*
  • Anti-Bacterial Agents / pharmacology
  • Chromatography, High Pressure Liquid
  • Honey / analysis*
  • Leptospermum / chemistry*
  • Microbial Viability / drug effects
  • Peroxides / chemistry
  • Pyruvaldehyde / chemistry


  • Anti-Bacterial Agents
  • Peroxides
  • Pyruvaldehyde