Protein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation

Metab Brain Dis. 2008 Mar;23(1):95-104. doi: 10.1007/s11011-007-9074-1. Epub 2008 Jan 16.


Protein phosphatase-2A (PP-2A) interacts with several regulators of cell death pathways and is therefore a potential component of signaling pathways linking global cerebral ischemia to cell death. Using a novel procedure to quantify PP-2A activity, we find that cardiac arrest with resuscitation and reperfusion leads to activation of PP-2A by 1.6-fold in pig brain extract and by 3.4-fold after partial purification of PP-2A. This is the first demonstration of PP-2A activation in a clinically relevant model of transient global cerebral ischemia. These results suggest that inhibition of PP-2A activity may be neuroprotective in global cerebral ischemia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / physiology
  • Brain / enzymology*
  • Brain Ischemia / enzymology
  • Cardiopulmonary Resuscitation*
  • Cell Death / physiology
  • Enzyme Activation / physiology
  • Enzyme Inhibitors / pharmacology
  • Heart Arrest / enzymology*
  • Necrosis / pathology
  • Protein Phosphatase 2 / antagonists & inhibitors
  • Protein Phosphatase 2 / isolation & purification
  • Protein Phosphatase 2 / metabolism*
  • Reperfusion Injury / enzymology
  • Swine


  • Enzyme Inhibitors
  • Protein Phosphatase 2