Overexpression of SPINDLIN1 induces cellular senescence, multinucleation and apoptosis

Gene. 2008 Feb 29;410(1):67-74. doi: 10.1016/j.gene.2007.11.019. Epub 2007 Dec 14.


Human or mouse Spindlin1 is expressed in various tissues and cells, but its biological functions are poorly understood. In this study, we show that human SPINDLIN1 is localized to interphase nucleus and mitotic chromosomes, and its expression in HeLa cells is not regulated in a cell cycle-dependent manner. When SPINDLIN1 is stably overexpressed in HeLa cells, it results in multinucleation of cells, and these multinucleated cells exhibits characteristic features of senescence and apoptosis shown by growth and morphological alterations, beta-galactosidase activity, and Annexin V/7-Aminoactinomycin D staining. Mouse Spindlin1 is highly homologous with human Spindlin1, when overexpressed in NIH3T3 cells, it also induces multinucleation, senescence and apoptosis in murine cells. Our results demonstrate that SPINDLIN1 is an important gene for mammalian mitotic chromosome functions, and disrupted regulation results in abnormal cell division, a mechanism that may be involved in tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis / genetics*
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics*
  • Cellular Senescence / genetics*
  • HeLa Cells
  • Humans
  • Mice
  • Microtubule-Associated Proteins / chemistry
  • Microtubule-Associated Proteins / genetics*
  • Molecular Sequence Data
  • NIH 3T3 Cells
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics*
  • Sequence Homology, Amino Acid


  • Cell Cycle Proteins
  • Microtubule-Associated Proteins
  • Phosphoproteins
  • spindlin