Characterization of interactions between and among components of the meiotic silencing by unpaired DNA machinery in Neurospora crassa using bimolecular fluorescence complementation

Genetics. 2008 Jan;178(1):593-6. doi: 10.1534/genetics.107.079384.

Abstract

Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa using two genes involved in meiotic silencing by unpaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Cell Nucleus / metabolism
  • DNA, Fungal / metabolism*
  • Fluorescence
  • Fungal Proteins / metabolism
  • Gene Silencing*
  • Luminescent Measurements / methods*
  • Luminescent Proteins / metabolism*
  • Meiosis*
  • Molecular Sequence Data
  • Neurospora crassa / cytology*
  • Neurospora crassa / metabolism*
  • Plasmids / genetics
  • Protein Binding
  • Protein Transport
  • Recombinant Fusion Proteins / metabolism*

Substances

  • Bacterial Proteins
  • DNA, Fungal
  • Fungal Proteins
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • yellow fluorescent protein, Bacteria