Targeted mRNA degradation by complex-mediated delivery of antisense RNAs to intracellular human mitochondria

Hum Mol Genet. 2008 May 1;17(9):1292-8. doi: 10.1093/hmg/ddn017. Epub 2008 Jan 18.


Mitochondrial dysfunction underlies a large number of acute or progressive diseases, as well as aging. However, proposed therapies for mitochondrial mutations suffer from poor transformation of mitochondria with exogenous DNA, or lack of functionality of the transferred nucleic acid within the organelle. We show that a transfer RNA import complex (RIC) from the parasitic protozoon Leishmania tropica rapidly and efficiently delivered signal-tagged antisense (STAS) RNA or DNA to mitochondria of cultured human cells. STAS-induced specific degradation of the targeted mitochondrial mRNA, with downstream effects on respiration. These results reveal the existence of a novel small RNA-mediated mRNA degradation pathway in mammalian mitochondria, and suggest that RIC-mediated delivery could be used to target therapeutic RNAs to the organelle within intact cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line, Tumor
  • Cell Respiration
  • Gene Targeting*
  • Humans
  • Leishmania tropica / genetics
  • Mitochondria / genetics*
  • Mitochondria / physiology
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism
  • RNA Stability*
  • RNA Transport
  • RNA, Antisense / chemistry
  • RNA, Antisense / genetics*
  • RNA, Antisense / physiology
  • RNA, Protozoan / genetics
  • RNA, Protozoan / isolation & purification
  • RNA, Protozoan / physiology
  • RNA, Transfer, Tyr / genetics*
  • RNA, Transfer, Tyr / isolation & purification
  • RNA, Transfer, Tyr / physiology


  • Mitochondrial Proteins
  • RNA, Antisense
  • RNA, Protozoan
  • RNA, Transfer, Tyr