Correlative expression of cyclooxygenase-1 (Cox-1) and human epidermal growth factor receptor type-2 (Her-2) in endometrial cancer

Kobe J Med Sci. 2007;53(5):177-87.


Objectives: Cyclooxygenase-2 (Cox-2) is known to be associated with tumorigenesis in many cancers including endometrial cancer, while there is substantial evidence for the tumorigenicity of cyclooxygenase-1 (Cox-1). However, little is known about the involvement of Cox-1 in the development of endometrial cancer. The aim of this study was to determine whether cyclooxygenase-1 or -2 (Cox-1, Cox-2) is tumorigenetic, as well as whether these two cyclooxygenase isoforms correlate with the clinicopathological characteristics or with another two biomarkers, human epidermal growth factor receptor type-2 (Her-2) and vascular endothelial growth factor (VEGF), of endometrial cancer.

Methods: At first, Cox-1 and Cox-2 levels in eight endometrial cancer cell lines were determined by means of real-time PCR. At second, the levels of four biomarkers (Cox-1, Cox-2, Her-2, and VEGF) in 70 endometrial cancer samples were determined by means of real-time PCR. Pairs of these biomarkers were subjected to correlation as each biomarker and clinical status or survival.

Results: In the eight cell lines, the expression of Cox-1 and Cox-2 showed major variations in their mRNA levels. Analysis of the patient samples showed that the mRNA expression of Cox-1 was elevated significantly in the G1 (P=0.021) and G2 (P=0.036) groups, as was the mRNA expression of Her-2 in the two groups (P=0.036 and P=0.0029, respectively). The mRNA expression of Cox-1 and Her-2 were correlated (CI=0.671). None of the three biomarkers, Cox-1, Cox-2, and Her-2, was correlated with clinical status such as FIGO classification, myometrial invasion, or clinical outcome.

Conclusion: Cox-1, together with Her-2, may be involved in the early stage of endometrial cancer development.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / enzymology*
  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology
  • Adult
  • Aged
  • Cell Line, Tumor
  • Cyclooxygenase 1 / biosynthesis
  • Cyclooxygenase 1 / genetics*
  • Cyclooxygenase 2 / biosynthesis
  • Cyclooxygenase 2 / genetics
  • Endometrial Neoplasms / enzymology*
  • Endometrial Neoplasms / metabolism
  • Endometrial Neoplasms / pathology
  • Female
  • Humans
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics
  • Middle Aged
  • RNA, Messenger / metabolism
  • Receptor, ErbB-2 / biosynthesis
  • Receptor, ErbB-2 / genetics*


  • Isoenzymes
  • RNA, Messenger
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • ERBB2 protein, human
  • Receptor, ErbB-2