Enzymatically catalyzed DNA synthesis using L-Asp-dGMP, L-Asp-dCMP, and L-Asp-dTMP

Chem Biodivers. 2008 Jan;5(1):31-9. doi: 10.1002/cbdv.200890013.

Abstract

The replacement of the pyrophosphate moiety of deoxynucleoside triphosphates by L-aspartic acid allows incorporation of natural deoxynucleosides into DNA using HIV reverse transcriptase (RT) as enzyme, while retaining the canonical base-pair selectivity. N-Methylation of the L-aspartic acid leaving group results in a reduced fidelity of incorporation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspartic Acid / chemistry*
  • Catalysis
  • DNA / chemical synthesis*
  • DNA / chemistry
  • Deoxycytidine Monophosphate / chemistry*
  • Deoxyguanine Nucleotides / chemistry*
  • HIV Reverse Transcriptase / chemistry*
  • Methylation
  • Molecular Conformation
  • Molecular Structure
  • Stereoisomerism
  • Thymidine Monophosphate / chemistry*

Substances

  • Deoxyguanine Nucleotides
  • Deoxycytidine Monophosphate
  • Aspartic Acid
  • Thymidine Monophosphate
  • 2'-deoxyguanosine 5'-phosphate
  • DNA
  • HIV Reverse Transcriptase