Genetic targeting of the endoderm with claudin-6CreER

Dev Dyn. 2008 Feb;237(2):504-12. doi: 10.1002/dvdy.21437.


A full description of the ontogeny of the beta cell would guide efforts to generate beta cells from embryonic stem cells (ESCs). The first step requires an understanding of definitive endoderm: the genes and signals responsible for its specification, proliferation, and patterning. This report describes a global marker of definitive endoderm, Claudin-6 (Cldn6). We report its expression in early development with particular attention to definitive endoderm derivatives. To create a genetic system to drive gene expression throughout the definitive endoderm with both spatial and temporal control, we target the endogenous locus with an inducible Cre recombinase (Cre-ER(T2)) cassette. Cldn6 null mice are viable and fertile with no obvious phenotypic abnormalities. We also report a lineage analysis of the fate of Cldn6-expressing embryonic cells, which is relevant to the development of the pancreas, lung, and liver.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Lineage / genetics
  • Cell Lineage / physiology*
  • Claudins
  • DNA Primers / genetics
  • Endoderm / embryology*
  • Endoderm / metabolism*
  • Galactosides
  • Gene Expression Regulation, Developmental / physiology*
  • Genotype
  • In Situ Hybridization
  • Indoles
  • Integrases / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • Mice, Knockout
  • Reverse Transcriptase Polymerase Chain Reaction


  • Claudins
  • DNA Primers
  • Galactosides
  • Indoles
  • Membrane Proteins
  • Cre recombinase
  • Integrases
  • claudin 6
  • 5-bromo-4-chloro-3-indolyl beta-galactoside