Virus detection and monitoring of viral load in Crimean-Congo hemorrhagic fever virus patients

Emerg Infect Dis. 2007 Jul;13(7):1097-100. doi: 10.3201/eid1307.070068.


We developed a real-time reverse transcription--PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genetic Variation*
  • Hemorrhagic Fever Virus, Crimean-Congo / classification
  • Hemorrhagic Fever Virus, Crimean-Congo / genetics
  • Hemorrhagic Fever Virus, Crimean-Congo / isolation & purification*
  • Hemorrhagic Fever, Crimean / blood
  • Hemorrhagic Fever, Crimean / diagnosis*
  • Hemorrhagic Fever, Crimean / mortality
  • Humans
  • Phylogeny
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Reverse Transcriptase Polymerase Chain Reaction / standards*
  • Sensitivity and Specificity
  • Species Specificity
  • Viral Load*