[The effect of staphylococcal alpha-toxin on DNA replication in human cells]

Tsitologiia. 1991;33(8):82-9.
[Article in Russian]

Abstract

The influence of Staphylococcus alpha-toxin has been investigated on the duration of S-phase of lymphocyte mitotic cycle and on DNA replication in human fibroblasts in vitro. The duration of the S-phase of lymphocytes was measured by counting labeled metaphases and by making replication curves. Alpha-toxin in a dose of 3 micrograms/ml enhances the onset of S-phase, which is inhibited at a dose of 33 micrograms/ml of alpha-toxin. The action of alpha-toxin resulted in a decreased rate of replication fork and in a progressive activation of replicon groups. This effect was most prominent at 33 micrograms/ml of alpha-toxin. The data obtained allow to suggest that immunodeficiency of the second order, so characteristic of the staphylococcal sepsis, may be due, in many respects, to suppression of DNA replication.

Publication types

  • English Abstract

MeSH terms

  • Autoradiography
  • Bacterial Toxins / pharmacology*
  • Cells, Cultured / drug effects
  • Cells, Cultured / metabolism
  • DNA / biosynthesis
  • DNA / drug effects*
  • DNA Replication / drug effects*
  • Dose-Response Relationship, Drug
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Hemolysin Proteins*
  • Humans
  • Lymphocytes / drug effects
  • Lymphocytes / metabolism
  • S Phase / drug effects
  • Staphylococcus aureus*

Substances

  • Bacterial Toxins
  • Hemolysin Proteins
  • staphylococcal alpha-toxin
  • DNA