Yeast and mammalian two-hybrid systems for studying protein-protein interactions

Methods Mol Biol. 2007;383:215-25. doi: 10.1007/978-1-59745-335-6_14.

Abstract

An important step in the analysis of protein function is identification of the interaction partners of each protein. The two-hybrid system has been widely used to identify and explore protein-protein interactions. By using various two-hybrid systems, numerous protein interactions that regulate apoptosis signaling have been discovered that reveal unexpected functions of cancer-relevant proteins. Methods for performing two-hybrid experiments using either yeast or mammalian cells will be described in this chapter.

MeSH terms

  • Animals
  • Humans
  • Lac Operon
  • Protein Binding
  • Protein Interaction Mapping
  • Proteins / metabolism*
  • Saccharomyces cerevisiae
  • Two-Hybrid System Techniques*

Substances

  • Proteins