Improving yeast two-hybrid screening systems

Brief Funct Genomic Proteomic. 2007 Dec;6(4):302-12. doi: 10.1093/bfgp/elm035. Epub 2008 Jan 24.


Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of protein-protein interactions. Optimisation and automation has increased the throughput of the method to an extent that allows the systematic mapping of protein-protein interactions on a proteome-wide scale. Since two-hybrid screens fail to detect a great number of interactions, parallel high-throughput approaches are needed for proteome-wide interaction screens. In this review, we discuss and compare different approaches for adaptation of Y2H screening to high-throughput, the limits of the method and possible alternative approaches to complement the mapping of organism-wide protein-protein interactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Artifacts
  • DNA, Complementary
  • Immunoprecipitation
  • Luciferases / metabolism
  • Protein Array Analysis
  • Two-Hybrid System Techniques*


  • DNA, Complementary
  • Luciferases