Brevibacterium marinum sp. nov., isolated from seawater

Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):500-4. doi: 10.1099/ijs.0.65099-0.


A novel yellow-pigmented actinobacterium was isolated from seawater collected from Hwasun Beach in Jeju, Republic of Korea. A comparative analysis of the 16S rRNA gene sequence indicated that the organism, designated HFW-26(T), was closely related to members of the genus Brevibacterium. As found for other species of the genus Brevibacterium, strain HFW-26(T) possessed meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, contained MK-8(H(2)) as the major menaquinone, contained polar lipids that included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid, and had anteiso-C(15 : 0) and anteiso-C(17 : 0) as the predominant fatty acids. The G+C content of the DNA was 71.4 mol%. The phylogenetically closest relative was Brevibacterium picturae DSM 16132(T) (99.0 % 16S rRNA gene sequence similarity). However, DNA-DNA hybridization of strain HFW-26(T) showed 35.1-43.7 % relatedness with respect to B. picturae DSM 16132(T). The novel isolate could be clearly distinguished from B. picturae DSM 16132(T) on the basis of some cultural, physiological and biochemical characteristics. A battery of phenotypic and genetic data obtained in this study suggest that strain HFW-26(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium marinum sp. nov. is proposed. The type strain is HFW-26(T) (=JBRI 2001(T)=KCTC 19221(T)=DSM 18964(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Brevibacterium / classification*
  • Brevibacterium / genetics
  • Brevibacterium / isolation & purification*
  • Brevibacterium / physiology
  • DNA, Bacterial / analysis
  • Fatty Acids / analysis
  • Genes, rRNA
  • Korea
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Species Specificity


  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AM421807