Attenuation of anaphylactic shock and related mortality in guinea-pigs after administration of a potent protein kinase inhibitor, K252a

J Basic Clin Physiol Pharmacol. Oct-Dec 1991;2(4):287-95. doi: 10.1515/jbcpp.1991.2.4.287.

Abstract

We examined whether protein kinases have a role in the expression of anaphylactic shock (AS). Guinea-pigs sensitized to ovalbumin were administered i.p. saline (control) or 10 micrograms/kg K252a, a potent protein kinase inhibitor, 30 min before challenge. The development of AS and mortality was observed for the next 2 h. In the K252a-treated group the incidence of AS fell to 53% from 100%, the maximum intensity was 62% less than the control, and mortality dropped to 16% from 50% of the animals. We suggest that protein kinases are involved in the expression of AS, and that inhibitors of these enzymes may protect against the symptoms of AS and allergy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphylaxis / enzymology*
  • Anaphylaxis / prevention & control
  • Animals
  • Carbazoles / pharmacology*
  • Guinea Pigs
  • Indole Alkaloids
  • Male
  • Phosphorylation
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism*

Substances

  • Carbazoles
  • Indole Alkaloids
  • staurosporine aglycone
  • Protein Kinase C