Sentinel node in melanoma patients: triple negativity with routine techniques and PCR as positive prognostic factor for survival

Mod Pathol. 2008 Apr;21(4):438-44. doi: 10.1038/modpathol.3801020. Epub 2008 Jan 25.


Lymph node mapping and sentinel lymph node biopsy are currently used to stage patients with cutaneous malignant melanoma. Immunohistochemical stains contribute to the detection of micrometastases; however, molecular biology techniques are associated with better diagnostic sensitivity. Sixty sentinel lymph nodes were included in this study. The primary lesions were malignant melanoma stage I or II, with a follow-up of longer than 2 years. Sentinel lymph nodes were studied with hematoxylin-eosin, immunohistochemistry for S-100 and HMB-45, and molecular biology techniques (reverse transcription (RT)-PCR) for the detection of tyrosinase messenger RNA. In 15 of 60 cases (25%), tyrosinase was detected by RT-PCR; three of these cases were also positive by immunohistochemistry. The population was divided into three groups: (i) hematoxylin-eosin-/immunohistochemistry+/molecular biology techniques+ (3 cases); (ii) hematoxylin-eosin-/immunohistochemistry-/molecular biology techniques+ (12 cases); (iii) hematoxylin-eosin-/immunohistochemistry-/molecular biology techniques- (45 cases). Correlation of the groups with overall survival showed the following: (i) 2 of 3 patients died (67%); (ii) 5 of 12 died (42%), and (iii) all 45 patients are alive, with no lymphadenectomy and a median follow-up of 84 months. The inclusion of molecular biology techniques appears to be of great value for the detection of sentinel lymph node micrometastases in patients with cutaneous malignant melanoma. In our series, those patients who showed negativity with all the three methods had a null recurrence rate. Therefore, this triple negativity could be a positive prognostic factor for overall survival. Our findings suggest the possibility of molecular oncological staging, which would allow the selection of patients with submicroscopic metastases for a complete treatment.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Neoplasm
  • Female
  • Humans
  • Immunohistochemistry
  • Lymph Nodes / metabolism
  • Lymph Nodes / pathology
  • Male
  • Melanoma / mortality
  • Melanoma / pathology*
  • Melanoma / surgery
  • Melanoma-Specific Antigens
  • Middle Aged
  • Monophenol Monooxygenase / biosynthesis
  • Monophenol Monooxygenase / genetics
  • Neoplasm Metastasis / diagnosis*
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Staging / methods*
  • Prognosis
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • S100 Proteins / biosynthesis
  • Sensitivity and Specificity
  • Sentinel Lymph Node Biopsy*
  • Skin Neoplasms / mortality
  • Skin Neoplasms / pathology*
  • Skin Neoplasms / surgery


  • Antigens, Neoplasm
  • Melanoma-Specific Antigens
  • Neoplasm Proteins
  • RNA, Messenger
  • S100 Proteins
  • Monophenol Monooxygenase