Abstract
By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems in leaf tissue to permit visualization of ER and Golgi dynamics in vivo by epifluorescence or confocal microscopy.
MeSH terms
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Cell Migration Assays / methods
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Cell Movement / physiology*
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Endoplasmic Reticulum / physiology
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Endoplasmic Reticulum / ultrastructure*
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Genetic Vectors / genetics
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Golgi Apparatus / physiology
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Golgi Apparatus / ultrastructure*
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Green Fluorescent Proteins / genetics*
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Microscopy, Confocal / methods
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Microscopy, Fluorescence / methods
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Molecular Biology / methods
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Plant Proteins / genetics
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Plant Viruses / genetics
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Plants / ultrastructure*
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Recombinant Fusion Proteins / genetics
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Rhizobium / genetics
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Staining and Labeling / methods*
Substances
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Plant Proteins
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Recombinant Fusion Proteins
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Green Fluorescent Proteins