Immunofluorescence staining

Curr Protoc Cell Biol. 2001 May;Chapter 4:Unit 4.3. doi: 10.1002/0471143030.cb0403s00.

Abstract

This unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. This provides both a visible signal and amplification of the signal and the results are observed with a fluorescent microscope.

MeSH terms

  • Animals
  • Antigen-Antibody Reactions
  • Cells, Cultured / chemistry
  • Cells, Cultured / ultrastructure
  • Fluorescent Antibody Technique, Indirect / methods*
  • Fluorescent Dyes / analysis
  • Indicators and Reagents
  • Microscopy, Fluorescence / methods*
  • Specimen Handling / methods
  • Staining and Labeling / methods*
  • Tissue Fixation / methods

Substances

  • Fluorescent Dyes
  • Indicators and Reagents