Abstract
Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods-cell-mediated dissolution of type I collagen fibrils, direct and reverse zymography, enzyme capture based on a-2 macroglubulin and TIMP-1 and -2, and demonstration of crytic thiol groups in metalloproteinase precursors-that are used to characterize the functions of matrix metalloproteinases and their inhibitors.
MeSH terms
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Animals
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Caseins / chemistry
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Collagen Type I / chemistry
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Cysteine / chemistry
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Enzyme Precursors / chemistry
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Enzyme Precursors / isolation & purification
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Fluorescent Dyes / chemistry
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Gelatin / chemistry
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Matrix Metalloproteinase Inhibitors
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Matrix Metalloproteinases / chemistry*
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Matrix Metalloproteinases / isolation & purification*
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Methods
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Rats
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Substrate Specificity
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Tissue Inhibitor of Metalloproteinase-1 / chemistry*
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Tissue Inhibitor of Metalloproteinase-2 / chemistry*
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alpha-Macroglobulins / chemistry*
Substances
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Caseins
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Collagen Type I
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Enzyme Precursors
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Fluorescent Dyes
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Matrix Metalloproteinase Inhibitors
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Tissue Inhibitor of Metalloproteinase-1
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alpha-Macroglobulins
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Tissue Inhibitor of Metalloproteinase-2
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Gelatin
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Matrix Metalloproteinases
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Cysteine