miR-125b Inhibits Osteoblastic Differentiation by Down-Regulation of Cell Proliferation

Biochem Biophys Res Commun. 2008 Apr 4;368(2):267-72. doi: 10.1016/j.bbrc.2008.01.073. Epub 2008 Jan 28.

Abstract

Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Line
  • Cell Proliferation
  • Down-Regulation
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism*
  • Mice
  • MicroRNAs / metabolism*
  • Osteoblasts / cytology*
  • Osteoblasts / metabolism*
  • Osteogenesis / physiology*

Substances

  • MicroRNAs
  • Mirn125 microRNA, mouse