Regulation of Interferon Signaling by the C and V Proteins From Attenuated and Wild-Type Strains of Measles Virus

Virology. 2008 Apr 25;374(1):71-81. doi: 10.1016/j.virol.2007.12.031. Epub 2008 Jan 29.

Abstract

The C and V proteins of the measles virus (MV) have been shown to block the signaling of type I and II interferon (IFN-alpha/beta and IFN-gamma). The relative contribution of the C and V proteins to the inhibition of IFN signaling and the extent to which this activity differs in attenuated or wild-type strains of MV remains undefined. This study presents a comparison of the IFN-antagonist activities of C and V proteins from four attenuated and two wild-type strains of MV. The V proteins were more potent inhibitors of IFN-inducible reporter gene expression than the C proteins, and this effect was unrelated to whether the protein originated from an attenuated or wild-type strain. The results also demonstrated the importance of the tyrosine at position 110 in the inhibition of IFN-alpha/beta and IFN-gamma signaling by the V protein, and identified a non-recombinant MV expressing a V protein that was impaired due to a mutation at this residue.

MeSH terms

  • Amino Acid Substitution
  • Artificial Gene Fusion
  • Genes, Reporter
  • Interferons / antagonists & inhibitors*
  • Luciferases / biosynthesis
  • Luciferases / genetics
  • Measles virus / genetics
  • Measles virus / immunology*
  • Molecular Sequence Data
  • Mutation, Missense
  • Phosphoproteins / immunology
  • Sequence Analysis, DNA
  • Tyrosine / genetics
  • Viral Proteins / genetics
  • Viral Proteins / immunology*

Substances

  • Phosphoproteins
  • V protein, measles virus
  • Viral Proteins
  • Tyrosine
  • Interferons
  • Luciferases

Associated data

  • GENBANK/EU026136