Direct immunoperoxidase staining for Regan isoenzyme of alkaline phosphatase in human tumor tissues

Cancer. 1976 Sep;38(3):1237-46. doi: 10.1002/1097-0142(197609)38:3<1237::aid-cncr2820380325>3.0.co;2-2.

Abstract

Immunoperoxidase staining for Regan isoenzyme of alkaline phosphatase was performed on cryostat sections of five human tumor tisssues. With a direct immunoperoxidase staining for the localization of Regan isoenzyme at the light and electron microscope levels, sections previously fixed with 0.05 M phosphate-buffered 4% paraformaldehyde were reacted with rabbit antisera to human placenta alkaline phosphatase conjugated to horseradish peroxidase. Comparison of conventional histochemistry and immunohistochemistry for Regan isoenzyme indicated that strong specific immunoperoxidase staining appeared on the cell membrane surface, and a diffuse one, in the cytoplasm of lung and colon cancer tissue cells showing L-phenylalanine-sensitive alkaline phosphatase. No immunoperoxidase reaction was obtained in tumor cells showing sensitivity to L-homoarginine or lacking aklaline phosphatase activity.

Publication types

  • Case Reports
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma
  • Aged
  • Alkaline Phosphatase / analysis*
  • Breast Neoplasms / enzymology
  • Carcinoma, Intraductal, Noninfiltrating / enzymology
  • Cell Membrane / enzymology
  • Cell Nucleus / enzymology
  • Colonic Neoplasms / enzymology
  • Female
  • Horseradish Peroxidase
  • Humans
  • Immunologic Techniques
  • Isoenzymes / analysis*
  • Lung Neoplasms / enzymology
  • Male
  • Microscopy, Electron
  • Middle Aged
  • Neoplasms / enzymology*
  • Neoplasms / pathology

Substances

  • Isoenzymes
  • Horseradish Peroxidase
  • Alkaline Phosphatase