Practical application of different enzymes immobilized on sepabeads

Bioprocess Biosyst Eng. 2008 Apr;31(3):163-71. doi: 10.1007/s00449-008-0199-3. Epub 2008 Feb 1.


The immobilization of an endoglucanase, benzoylformate decarboxylase (BFD) from Pseudomonas putida, as well as of lipase B from Candida antarctica (CALB) onto the carrier supports Sepabeads EC-EP, Sepabeads EC-EA, and Sepabeads EC-BU was accomplished. It is shown that via these immobilized biocatalysts the synthesis of both fine and bulk chemicals is possible. This is illustrated by the syntheses of polyglycerol esters and (S)-hydroxy phenyl propanone. The benefit of immobilization is illustrated by repetitive use in a bubble column reactor as well as in a stirred tank reactor. High stability of two biocatalysts was achieved and reusability up to eight times was demonstrated. The comparison of CALB immobilized on Sepabeads EC-EP to Novozym 435 shows similar activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Biotechnology / methods*
  • Candida / enzymology
  • Catalysis
  • Cellulase / chemistry
  • Chemistry / methods
  • Enzymes / chemistry
  • Enzymes, Immobilized / chemistry*
  • Escherichia coli / enzymology
  • Esters / chemistry
  • Kinetics
  • Pseudomonas putida / enzymology
  • Solvents / chemistry
  • Temperature
  • Time Factors


  • Enzymes
  • Enzymes, Immobilized
  • Esters
  • Solvents
  • Cellulase