Evidence for GC preference by monocot Dicer-like proteins

Biochem Biophys Res Commun. 2008 Apr 4;368(2):433-7. doi: 10.1016/j.bbrc.2008.01.110. Epub 2008 Feb 1.


Dicot Dicer-like (DCL) enzymes operate preferably on GC rich regions when producing small interfering (si)RNA and micro (mi)RNA. This GC bias, however, is not generic in monocot miRNA productions. From wild Dactylis glomerata naturally infected by Cocksfoot streak potyvirus (CSV), CSV-siRNAs had a greater GC% than the virus genome, indicating that GC rich regions were also preferred by the grass DCLs. This supports the notion that GC preference is an ancient feature for plant DCLs, and suggests that monocot miRNA genes might have evolved to a high GC% resulting in GC bias being not detectable during mature miRNA production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition / genetics*
  • Base Sequence
  • DNA, Plant / genetics*
  • Dactylis / genetics*
  • Evolution, Molecular*
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • RNA, Small Interfering / genetics*
  • Ribonuclease III / genetics*


  • DNA, Plant
  • Plant Proteins
  • RNA, Small Interfering
  • Ribonuclease III