CTA1-M2e-DD: A Novel Mucosal Adjuvant Targeted Influenza Vaccine

Vaccine. 2008 Feb 26;26(9):1243-52. doi: 10.1016/j.vaccine.2007.12.027. Epub 2008 Jan 10.

Abstract

At present few vaccine candidates exists against potentially pandemic influenza virus infections. We provide compelling evidence that a targeted fusion protein based on the CTA1-DD adjuvant and containing tandem repeats of the matrix protein 2 (M2e) ectodomain epitope, CTA1-3M2e-DD, confers strong protective immunity against a potentially lethal challenge infection with influenza virus in mice. The formulation was highly effective for mucosal immunizations and promoted high M2e-specific serum IgG and mucosal IgA antibody titers and an hitherto unknown anti-M2e CD4 T cell immunity. This novel CTA1-3M2e-DD fusion protein combines adjuvant and a conserved influenza A antigen in a promising candidate for a universal anti-influenza vaccine.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage*
  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / blood
  • B-Lymphocytes / immunology
  • Cholera Toxin / administration & dosage
  • Cholera Toxin / immunology*
  • Female
  • Immunity, Mucosal*
  • Immunization
  • Immunoglobulin A / analysis
  • Immunoglobulin G / blood
  • Influenza A Virus, H1N1 Subtype / genetics
  • Influenza A Virus, H1N1 Subtype / pathogenicity
  • Influenza A Virus, H3N2 Subtype / genetics
  • Influenza A Virus, H3N2 Subtype / pathogenicity
  • Influenza Vaccines / administration & dosage
  • Influenza Vaccines / genetics
  • Influenza Vaccines / immunology*
  • Male
  • Mice
  • Molecular Sequence Data
  • Orthomyxoviridae Infections / immunology*
  • Orthomyxoviridae Infections / prevention & control
  • Reassortant Viruses / genetics
  • Reassortant Viruses / pathogenicity
  • Recombinant Fusion Proteins / administration & dosage
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology*
  • Viral Matrix Proteins / administration & dosage
  • Viral Matrix Proteins / chemistry
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / immunology*

Substances

  • Adjuvants, Immunologic
  • Antibodies, Viral
  • CTA1-DD protein, recombinant
  • Immunoglobulin A
  • Immunoglobulin G
  • Influenza Vaccines
  • M2 protein, Influenza A virus
  • M2e-HBc influenza vaccine
  • Recombinant Fusion Proteins
  • Viral Matrix Proteins
  • Cholera Toxin