Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene

Appl Environ Microbiol. 2008 Mar;74(6):1941-4. doi: 10.1128/AEM.01658-07. Epub 2008 Feb 1.

Abstract

A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacteria / classification
  • Bacteria / genetics*
  • Bacteria / metabolism
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Oxidoreductases / genetics*
  • Oxidoreductases / metabolism
  • Perchlorates / metabolism*
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Soil Microbiology

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Perchlorates
  • Oxidoreductases
  • perchlorate

Associated data

  • GENBANK/EU273890
  • GENBANK/EU273891
  • GENBANK/EU273892
  • GENBANK/EU273893
  • GENBANK/EU273894
  • GENBANK/EU273895
  • GENBANK/EU273896
  • GENBANK/EU273897
  • GENBANK/EU273898