Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2008 Feb 1;22(3):279-85.
doi: 10.1101/gad.1643108.

The search for alternative splicing regulators: new approaches offer a path to a splicing code

Charles J David  1 , James L Manley
Affiliations
Comment

The search for alternative splicing regulators: new approaches offer a path to a splicing code

Charles J David et al. Genes Dev. .
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
ASD-2b promotes E10 inclusion by altering 5′ splice site usage in the intron downstream from the regulated exons. In embryos, E10 is removed in a fast splicing event, leaving a product with E9 and E11 ligated together as the predominant partially spliced isoform, which is processed to remove intron 8 in a slower reaction. In adults, ASD-2b, through an unknown mechanism, promotes usage of the E10 donor site in the fast reaction. This results in a competition between E9 and E10 acceptor sites. E9 is removed because it lies downstream from a weaker 3′ splice site.
Figure 2.
Figure 2.
Potential mechanisms for exon inclusion stimulated by AS regulators binding to the downstream intron. In the recruitment pathway, the intron-bound AS regulator promotes exon recognition through specific contacts with the splicing machinery. TIA1 recruitment of U1 snRNP to 5′ splice sites of exons that it regulates serves as an example. In the conformational pathway, regulators bound to an intronic site promote regulated exon inclusion by intron definition. This might involve an interaction between regulators bound at either end of the intron that would serve to loop out intronic sequences and bring the regulated exon into proximity with the downstream constitutive exon, thereby favoring its inclusion.
See this image and copyright information in PMC

Comment on

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Allemand E., Guil S., Myers M., Moscat J., Caceres J.F., Krainer A.R. Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock. Proc. Natl. Acad. Sci. 2005;102:3605–3610. - PMC - PubMed
    1. Anyanful A., Ono K., Johnsen R.C., Ly H., Jensen V., Baillie D.L., Ono S. The RNA-binding protein SUP-12 controls muscle-specific splicing of the ADF/cofilin pre-mRNA in C. elegans. J. Cell Biol. 2004;167:639–647. - PMC - PubMed
    1. Auweter S.D., Fasan R., Reymond L., Underwood J.G., Black D.L., Pitsch S., Allain F.H. Molecular basis of RNA recognition by the human alternative splicing factor Fox-1. EMBO J. 2006;25:163–173. - PMC - PubMed
    1. Bharadwaj R., Kolodkin A.L. Descrambling Dscam diversity. Cell. 2006;125:421–424. - PubMed
    1. Black D.L. Activation of c-src neuron-specific splicing by an unusual RNA element in vivo and in vitro. Cell. 1992;69:795–807. - PubMed

MeSH terms

Substances

LinkOut - more resources