Adenovirus type 5 E3 gene products interfere with the expression of the cytolytic T cell immunodominant E1a antigen

Virology. 1991 Jan;180(1):199-206. doi: 10.1016/0042-6822(91)90024-6.

Abstract

Effects of mutations in the adenovirus 5 (Ad 5) E3 transcription unit on the immune Tc cell response to Ad 5 were investigated. We observed enhanced lysis of L929 target cells infected with the E3 defective mutant viruses dl 327 and dl 355 compared to wild-type (wt) Ad 5 by Ad 5 immune Tc cells. This enhanced lysability was not due to E3 effects on the cell surface expression of class I MHC H-2Kk molecules as determined by monoclonal antibody binding or alloreactive Tc cell recognition. Furthermore MHC class I molecules were able to efficiently present vaccinia virus antigens in the presence of the Ad 5 E3 genes, excluding functional modification of class I MHC antigens by E3 gene products. When levels of the Ad 5 immunodominant antigen E1a were compared between wt and E3 mutant viruses, we observed an 8- to 10-fold increase in E1a levels in E3 mutant-infected cells over wt Ad 5-infected cells. No differences were observed between these viruses at the mRNA level. We conclude that E3 products interfere with Ad 5 immune Tc cell responses by some post-transcriptional mechanism which reduces expression of the E1a immunodominant antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus Early Proteins
  • Adenoviruses, Simian / genetics*
  • Adenoviruses, Simian / immunology
  • Adenoviruses, Simian / physiology
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, Viral / immunology
  • Cytotoxicity, Immunologic / immunology
  • Genes, Viral*
  • H-2 Antigens / biosynthesis
  • H-2 Antigens / immunology
  • Immunodominant Epitopes / immunology
  • Mice
  • Mice, Inbred CBA
  • Mutation
  • Oncogene Proteins, Viral / biosynthesis
  • Oncogene Proteins, Viral / immunology*
  • RNA, Messenger / biosynthesis
  • RNA, Viral / biosynthesis
  • T-Lymphocytes, Cytotoxic / immunology*
  • Transcription, Genetic / genetics
  • Vaccinia virus / immunology
  • Viral Proteins*

Substances

  • Adenovirus Early Proteins
  • Antibodies, Monoclonal
  • Antigens, Viral
  • H-2 Antigens
  • H-2K(K) antigen
  • Immunodominant Epitopes
  • Oncogene Proteins, Viral
  • RNA, Messenger
  • RNA, Viral
  • Viral Proteins