A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae

Nat Methods. 2008 Mar;5(3):239-41. doi: 10.1038/nmeth.1181. Epub 2008 Feb 3.


Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Gene Expression Profiling / methods*
  • Gene Expression Regulation, Fungal
  • Gene Library*
  • Genes, Fungal / genetics
  • Genome, Fungal / genetics*
  • Saccharomyces cerevisiae / genetics*