Selenoprotein A component of the glycine reductase complex from Clostridium purinolyticum: nucleotide sequence of the gene shows that selenocysteine is encoded by UGA

J Bacteriol. 1991 Mar;173(6):2093-8. doi: 10.1128/jb.173.6.2093-2098.1991.

Abstract

The gene encoding the selenoprotein A component of glycine reductase was isolated from Clostridium purinolyticum. The nucleotide sequence of this gene (grdA) was determined. The opal termination codon (TGA) was found in-frame at the position corresponding to the location of the selenocysteine residue in the gene product. A comparison of the nucleotide sequences and secondary mRNA structures corresponding to the selenoprotein A gene and the fdhF gene of Escherichia coli formate dehydrogenase shows that there is a similar potential for regulation of the specific insertion of selenocysteine at the UGA codon.

MeSH terms

  • Amino Acid Oxidoreductases / genetics*
  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Clostridium / genetics*
  • Codon
  • Cysteine / analogs & derivatives*
  • Cysteine / genetics
  • Genes, Bacterial
  • Molecular Sequence Data
  • Molecular Structure
  • Multienzyme Complexes / genetics*
  • Nucleic Acid Conformation
  • RNA, Messenger / ultrastructure
  • Restriction Mapping
  • Selenium
  • Selenocysteine
  • Selenoproteins

Substances

  • Bacterial Proteins
  • Codon
  • Multienzyme Complexes
  • RNA, Messenger
  • Selenoproteins
  • selenoprotein A
  • Selenocysteine
  • Amino Acid Oxidoreductases
  • glycine reductase
  • Selenium
  • Cysteine

Associated data

  • GENBANK/M55254