Abstract
The transcriptional response to auxin is critical for root and vascular development during Arabidopsis embryogenesis. Auxin induces the degradation of AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors, freeing their binding partners, the AUXIN RESPONSE FACTOR (ARF) proteins, which can activate transcription of auxin response genes. We show that TOPLESS (TPL) can physically interact with IAA12/BODENLOS (IAA12/BDL) through an ETHYLENE RESPONSE FACTOR (ERF)-associated amphiphilic repression (EAR) motif. TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity. In addition, tpl-1 can suppress the patterning defects of the bdl-1 mutant. Direct interaction between TPL and ARF5/MONOPTEROS, which is regulated by IAA12/BDL, results in a loss-of-function arf5/mp phenotype. These observations show that TPL is a transcriptional co-repressor and further our understanding of how auxin regulates transcription during plant development.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Motifs
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Arabidopsis / embryology
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Plant*
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Indoleacetic Acids / metabolism*
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Models, Genetic
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Mutation
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Protein Binding
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Seedlings / embryology
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Seedlings / metabolism
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Seeds / embryology
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Seeds / metabolism
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Transcription Factors / metabolism
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Transcription, Genetic*
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Two-Hybrid System Techniques
Substances
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Arabidopsis Proteins
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DNA-Binding Proteins
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IAA12 protein, Arabidopsis
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Indoleacetic Acids
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MONOPTEROS protein, Arabidopsis
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Recombinant Fusion Proteins
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Repressor Proteins
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Transcription Factors
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topless protein, Arabidopsis