In vitro regulation of B cell differentiation by interleukin-6 and soluble CD23 in systemic lupus erythematosus B cell subpopulations and antigen-induced normal B cells

Arthritis Rheum. 1991 Mar;34(3):276-86. doi: 10.1002/art.1780340305.

Abstract

Polyreactive systemic lupus erythematosus (SLE) B cells were compared with antigen-induced SLE and normal B cells for their interleukin-6 (IL-6) and soluble CD23 requirements. Unlike normal B cells, secretion of antibody by SLE B cells in serum-free medium was not enhanced by exogenous IL-6. Anti-IL-6 antibodies inhibited immunoglobulin production in cultures of normal and SLE B cells, which suggests that IL-6 is required for B cell differentiation. SLE culture supernatants had elevated levels of IL-6, which explains the poor response of the SLE cells to exogenous IL-6. Soluble CD23 enhanced the responses of cells from normal subjects and SLE patients.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Antibody Formation
  • Antigens, CD / pharmacology*
  • Antigens, Differentiation, B-Lymphocyte / pharmacology*
  • B-Lymphocyte Subsets / pathology
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • B-Lymphocytes / pathology*
  • Cell Differentiation
  • Female
  • Humans
  • Immunization
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / pharmacology*
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • Receptors, Fc / pharmacology*
  • Receptors, IgE
  • Solubility
  • Tetanus Toxoid / immunology*

Substances

  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • Interleukin-6
  • Receptors, Fc
  • Receptors, IgE
  • Tetanus Toxoid