Strain-specific inhibition of Helicobacter pylori by Lactobacillus salivarius and other lactobacilli

J Antimicrob Chemother. 2008 Apr;61(4):831-4. doi: 10.1093/jac/dkn040. Epub 2008 Feb 8.


Objectives: To investigate the anti-Helicobacter pylori activity of 28 strains of Lactobacillus salivarius and 12 other lactobacilli, isolated from different sites and from different geographical regions.

Methods: An in vitro agar plate diffusion assay was employed to assess the Lactobacillus anti-H. pylori activity.

Results: Nine out of 28 L. salivarius strains and 3/12 other Lactobacillus species tested inhibited H. pylori growth. There was no correlation between ecological niche/geographical location of isolation of the lactobacilli and their inhibitory capability. Further studies on strain L. salivarius UCC119 showed that this strain could inhibit growth of 6/6 clinical isolates of H. pylori, five of which were antibiotic-resistant. This inhibition was not due to acid production and was not mediated by a protein, but did require the presence of live cells.

Conclusions: Growth inhibition of H. pylori by L. salivarius is strain-dependent and is not linked to any particular environmental niche or geographic location. Strains of L. salivarius showing highest anti-H. pylori activity may be useful as an adjunct in the treatment of strains that are resistant to conventional antibiotics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiosis*
  • Bacterial Proteins / metabolism
  • Culture Media / chemistry
  • Helicobacter Infections / microbiology
  • Helicobacter pylori / growth & development*
  • Humans
  • Hydrogen-Ion Concentration
  • Lactobacillus / physiology*


  • Bacterial Proteins
  • Culture Media